Glutamate receptor exocytosis and spine enlargement during chemically induced long-term potentiation

Kopec, C. D., Li, B., Wei, W., Boehm, J., Malinow, R. (February 2006) Glutamate receptor exocytosis and spine enlargement during chemically induced long-term potentiation. J Neurosci, 26 (7). pp. 2000-9. ISSN 1529-2401 (Electronic)

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DOI: 10.1523/jneurosci.3918-05.2006


The changes in synaptic morphology and receptor content that underlie neural plasticity are poorly understood. Here, we use a pH-sensitive green fluorescent protein to tag recombinant glutamate receptors and monitor their dynamics onto dendritic spine surfaces. We show that chemically induced long-term potentiation (chemLTP) drives robust exocytosis of AMPA receptors. In contrast, the same stimulus produces a small reduction of NMDA receptors from the spine surface. chemLTP produces similar modification of small and large spines. Interestingly, during chemLTP induction, spines increase in volume before accumulation of AMPA receptors on their surface, indicating that distinct mechanisms underlie changes in morphology and receptor content.

Item Type: Paper
Uncontrolled Keywords: Animals Exocytosis physiology Hippocampus physiology Long Term Potentiation drug effects physiology Rats Receptors AMPA physiology Receptors Glutamate physiology Spine physiology Synapses drug effects physiology Transfection
Subjects: organs, tissues, organelles, cell types and functions > cell types and functions > cell functions > neural plasticity
CSHL Authors:
Communities: CSHL labs > Malinow lab
School of Biological Sciences > Publications
Depositing User: Editor Margaret Fantz
Date: 15 February 2006
Date Deposited: 24 Feb 2012 16:37
Last Modified: 19 Sep 2014 18:44
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