Kopec, C. D., Li, B., Wei, W., Boehm, J., Malinow, R. (February 2006) Glutamate receptor exocytosis and spine enlargement during chemically induced long-term potentiation. J Neurosci, 26 (7). pp. 2000-9. ISSN 1529-2401 (Electronic)
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Abstract
The changes in synaptic morphology and receptor content that underlie neural plasticity are poorly understood. Here, we use a pH-sensitive green fluorescent protein to tag recombinant glutamate receptors and monitor their dynamics onto dendritic spine surfaces. We show that chemically induced long-term potentiation (chemLTP) drives robust exocytosis of AMPA receptors. In contrast, the same stimulus produces a small reduction of NMDA receptors from the spine surface. chemLTP produces similar modification of small and large spines. Interestingly, during chemLTP induction, spines increase in volume before accumulation of AMPA receptors on their surface, indicating that distinct mechanisms underlie changes in morphology and receptor content.
| Item Type: | Paper |
|---|---|
| Uncontrolled Keywords: | Animals Exocytosis physiology Hippocampus physiology Long Term Potentiation drug effects physiology Rats Receptors AMPA physiology Receptors Glutamate physiology Spine physiology Synapses drug effects physiology Transfection |
| Subjects: | organs, tissues, organelles, cell types and functions > cell types and functions > cell functions > neural plasticity |
| CSHL Authors: | |
| Communities: | CSHL labs > Malinow lab School of Biological Sciences > Publications |
| Depositing User: | Editor Margaret Fantz |
| Date: | 15 February 2006 |
| Date Deposited: | 24 Feb 2012 16:37 |
| Last Modified: | 19 Sep 2014 18:44 |
| Related URLs: | |
| URI: | https://repository.cshl.edu/id/eprint/24901 |
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