Comparison of tangential flow filtration and centrifugation for monoclonal antibody harvest clarification

Schroeder, J. B., Krul, A., Riske, F.J. (March 2004) Comparison of tangential flow filtration and centrifugation for monoclonal antibody harvest clarification. In: ACS National Convention, Anaheim CA..



Two candidate technologies, Tangential flow filtration, and centrifugation followed by depth filtration, were evaluated for the initial clarification of fed batch harvest containing a monoclonal antibody. The goal was to evaluate and compare the two technologies so that one of the technologies could be chosen for use at full scale. Optimal operating parameters for both systems were determined using scale-down systems. Volume end-point (Vmax) method using 0.2 and 0.45 um filters was developed to evaluate the quality of the clarified product. Product breakthrough endpoint (Pmax) testing was utilized to evaluate depth filters to be used in conjunction with centrifugation. Based on the results of these studies, and consideration of factors such as step yield, buffer usage, CIP / SIP, man power requirements, maintenance, robustness, and other economic factors, centrifugation with depth filtration was chosen for the process at scale.

Item Type: Conference or Workshop Item (Paper)
Additional Information: Poster
Uncontrolled Keywords: Biotechnology depth filtration continuous flow centrifugation monoclonal antibody purification Tangential flow filtration (TFF) Vmax Pmax
Subjects: Investigative techniques and equipment > Pmax
Investigative techniques and equipment > Vmax
biotechnology > initial clarification > bioreactor harvest tangential flow filtration
biotechnology > initial clarification > continuous feed centrifugation
biotechnology > filtration > depth filtration
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > monoclonal antibody
CSHL Authors:
Depositing User: CSHL Librarian
Date: 28 March 2004
Date Deposited: 06 Dec 2011 22:32
Last Modified: 06 Dec 2011 22:32

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