Using three-dimensional acinar structures for molecular and cell biological assays

Xiang, B., Muthuswamy, S. K. (2006) Using three-dimensional acinar structures for molecular and cell biological assays. Methods Enzymol, 406. pp. 692-701. ISSN 0076-6879 (Print)

DOI: 10.1016/s0076-6879(06)06054-x


Epithelial cells grown in three-dimensional matrix allows one to investigate biological processes in a setting that is closer to in vivo conditions than those obtained by growing cells on plastic culture dishes. Here we outline procedures that allow one to investigate molecular mechanisms that regulate formation and disruption of three-dimensional epithelial structures.

Item Type: Paper
Uncontrolled Keywords: Cell Culture Techniques methods Collagen ultrastructure Cyclin-Dependent Kinase metabolism Cyclin-Dependent Kinase 4 metabolism Drug Combinations Epithelial Cells ultrastructure Fluorescent Antibody Technique, Indirect Humans Laminin ultrastructure Proteoglycans ultrastructure
Subjects: Investigative techniques and equipment > cell culture
organs, tissues, organelles, cell types and functions > tissues types and functions > epithelia
CSHL Authors:
Communities: CSHL labs > Muthuswamy lab
Depositing User: CSHL Librarian
Date: 2006
Date Deposited: 02 Dec 2011 19:07
Last Modified: 30 Apr 2018 16:42
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