Xiang, B., Muthuswamy, S. K. (2006) Using three-dimensional acinar structures for molecular and cell biological assays. Methods Enzymol, 406. pp. 692-701. ISSN 0076-6879 (Print)
Abstract
Epithelial cells grown in three-dimensional matrix allows one to investigate biological processes in a setting that is closer to in vivo conditions than those obtained by growing cells on plastic culture dishes. Here we outline procedures that allow one to investigate molecular mechanisms that regulate formation and disruption of three-dimensional epithelial structures.
| Item Type: | Paper |
|---|---|
| Uncontrolled Keywords: | Cell Culture Techniques methods Collagen ultrastructure Cyclin-Dependent Kinase metabolism Cyclin-Dependent Kinase 4 metabolism Drug Combinations Epithelial Cells ultrastructure Fluorescent Antibody Technique, Indirect Humans Laminin ultrastructure Proteoglycans ultrastructure |
| Subjects: | Investigative techniques and equipment > cell culture organs, tissues, organelles, cell types and functions > tissues types and functions > epithelia |
| CSHL Authors: | |
| Communities: | CSHL labs > Muthuswamy lab |
| Depositing User: | CSHL Librarian |
| Date: | 2006 |
| Date Deposited: | 02 Dec 2011 19:07 |
| Last Modified: | 30 Apr 2018 16:42 |
| Related URLs: | |
| URI: | https://repository.cshl.edu/id/eprint/22938 |
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