Woolcock, B., Kazianis, S., Lucito, R., Walter, R. B., Kallman, K. D., Morizot, D. C., Vielkind, J. R. (2006) Allele-specific marker generation and linkage mapping on the Xiphophorus sex chromosomes. Zebrafish, 3 (1). pp. 23-37. ISSN 15458547
Abstract
There is great interest in the sex chromosomes of Xiphophorus fishes because both WY/YY and XX/XY sex-determining mechanisms function in these species, with at least one taxon possessing all three types of sex chromosomes, and because in certain interspecific hybrids melanoma arises as a consequence of inheritance of the sex-linked macromelanophore determining locus (MDL). Representational difference analysis (RDA) has been used to clone two sequences from the sex-determining region of X. maculatus, including a cholinergic receptor, nicotinic, delta polypeptide (CHRND) orthologue. Allele-specific assays for these sequences, as well as for the sex-linked XMRK1 and XMRK2 genes, were developed to distinguish W, X, and Y chromosomes derived from a X. maculatus (XX/XY) strain and a X. helleri (WY/YY) strain. Linkage mapping localized these markers to linkage group (LG) 24. No recombinants were observed between XMRK2 and MDL, confirming a role for XMRK2 in macromelanophore development. Although the master sex-determining (SD) locus certainly resides on Xiphophorus LG 24, autosomal loci are probably involved in sex determination as well, as indicated by the abnormal sex ratios in the backcross1 hybrids that contrast theoretical predictions based on LG 24 genotyping. Marker development and allelic discrimination on the Xiphophorus sex chromosomes should prove highly useful for studies that utilize this genus as an animal model. © Mary Ann Liebert, Inc.
| Item Type: | Paper |
|---|---|
| Subjects: | bioinformatics > genomics and proteomics > annotation > sequence annotation bioinformatics > genomics and proteomics > Mapping and Rendering > Sequence Rendering diseases & disorders > cancer > cancer types > melanomas organism description > animal > fish > zebrafish |
| CSHL Authors: | |
| Communities: | CSHL labs > Lucito lab |
| Depositing User: | CSHL Librarian |
| Date: | 2006 |
| Date Deposited: | 08 Dec 2011 15:49 |
| Last Modified: | 30 Apr 2018 16:34 |
| Related URLs: | |
| URI: | https://repository.cshl.edu/id/eprint/22937 |
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