Principles of two-photon excitation microscopy and its applications to neuroscience

Svoboda, K., Yasuda, R. (June 2006) Principles of two-photon excitation microscopy and its applications to neuroscience. Neuron, 50 (6). pp. 823-39. ISSN 0896-6273 (Print)

DOI: 10.1016/j.neuron.2006.05.019


The brain is complex and dynamic. The spatial scales of interest to the neurobiologist range from individual synapses (approximately 1 microm) to neural circuits (centimeters); the timescales range from the flickering of channels (less than a millisecond) to long-term memory (years). Remarkably, fluorescence microscopy has the potential to revolutionize research on all of these spatial and temporal scales. Two-photon excitation (2PE) laser scanning microscopy allows high-resolution and high-sensitivity fluorescence microscopy in intact neural tissue, which is hostile to traditional forms of microscopy. Over the last 10 years, applications of 2PE, including microscopy and photostimulation, have contributed to our understanding of a broad array of neurobiological phenomena, including the dynamics of single channels in individual synapses and the functional organization of cortical maps. Here we review the principles of 2PE microscopy, highlight recent applications, discuss its limitations, and point to areas for future research and development.

Item Type: Paper
Uncontrolled Keywords: Animals Humans Microscopy Confocal instrumentation methods Microscopy, Fluorescence Multiphoton instrumentation methods Neurosciences instrumentation methods trends Photobleaching
Subjects: Investigative techniques and equipment > imaging > flourescence bleaching
Investigative techniques and equipment > microscopy > flourescence microscopy
Investigative techniques and equipment > microscopy
Investigative techniques and equipment > optical devices > lasers > two-photon excitation laser scanning
CSHL Authors:
Communities: CSHL labs > Svoboda lab
Depositing User: CSHL Librarian
Date: 15 June 2006
Date Deposited: 08 Dec 2011 18:25
Last Modified: 27 Apr 2018 19:33
Related URLs:

Actions (login required)

Administrator's edit/view item Administrator's edit/view item
CSHL HomeAbout CSHLResearchEducationNews & FeaturesCampus & Public EventsCareersGiving