Ruthazer, E. S., Li, J., Cline, H. T. (March 2006) Stabilization of axon branch dynamics by synaptic maturation. J Neurosci, 26 (13). pp. 3594-603. ISSN 1529-2401 (Electronic)
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Abstract
The developmental refinement of topographic projections in the brain is reflected in the dynamic sculpting of axonal arbors that takes place as connections between CNS structures form and mature. To examine the role of synaptogenesis and synaptic maturation in the structural development of axonal projections during the formation of the topographic retinotectal projection, we coexpressed cytosolic fluorescent protein (FP) and FP-tagged synaptophysin (SYP) in small numbers of retinal ganglion cells in living albino Xenopus laevis tadpoles to reveal the distribution and dynamics of presynaptic sites within labeled retinotectal axons. Two-photon time-lapse observations followed by quantitative analysis of tagged SYP levels at individual synapses demonstrated the time course of synaptogenesis: increases in presynaptic punctum intensity are detectable within minutes of punctum emergence and continue over many hours. Puncta lifetimes correlate with their intensities. Furthermore, we found that axon arbor dynamics are affected by synaptic contacts. Axon branches retract past faintly labeled puncta but are locally stabilized at intensely labeled SYP puncta. Visual stimulation for 4 h enhanced the stability of the arbor at intense presynaptic puncta while concurrently inducing the retraction of exploratory branches with only faintly labeled or no synaptic sites.
| Item Type: | Paper |
|---|---|
| Uncontrolled Keywords: | Aging pathology physiology Animals Axons physiology ultrastructure Cells, Cultured Nerve Net cytology physiology Neuronal Plasticity physiology Retinal Ganglion Cells cytology physiology Synapses physiology ultrastructure Xenopus laevis anatomy & histology physiology |
| Subjects: | bioinformatics > genomics and proteomics > Mapping and Rendering organs, tissues, organelles, cell types and functions > tissues types and functions > axon organs, tissues, organelles, cell types and functions > organs types and functions > brain organs, tissues, organelles, cell types and functions > sub-cellular tissues: types and functions > synapse organs, tissues, organelles, cell types and functions > tissues types and functions > synaptogenesis |
| CSHL Authors: | |
| Communities: | CSHL labs > Cline lab |
| Depositing User: | CSHL Librarian |
| Date: | 29 March 2006 |
| Date Deposited: | 08 Dec 2011 21:21 |
| Last Modified: | 06 Nov 2017 21:15 |
| Related URLs: | |
| URI: | https://repository.cshl.edu/id/eprint/22890 |
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