Muratani, Masafumi, Kung, Charles, Shokat, Kevan M., Tansey, William P. (March 2005) The F box protein Dsg1/Mdm30 is a transcriptional coactivator that stimulates Gal4 turnover and cotranscriptional mRNA processing. Cell, 120 (6). pp. 887-899. ISSN 0092-8674
Abstract
We report here that the prototypical yeast transcription factor Gal4 undergoes two distinct modes of ubiquitin-mediated proteolysis: one that occurs independent of transcription and restricts Gal4 function, and another that is transcription coupled and essential for productive activation of Gal4 target genes. Destruction of transcriptionally active Gal4 depends on an F box protein called Dsgl/Mdm30. In the absence of Dsg1, Gal4 is stable, nonubiquitylated, and unable to productively stimulate transcription. Analysis of the phenotype of dsgl-nuil yeast reveals a striking disconnect between GAL gene RNA and protein levels; in the absence of Dsgl, Gal4 target genes are transcribed, but the resulting RNAs are not translated. The translational defects of these RNAs are related to defects in phosphorylation of the RNA polymerase 11 carboxy-terminal domain, which in turn affects recruitment of RNA processing machinery. We propose that Gal4 ubiquitylation and destruction are required for initiation-competent transcription complexes to transition to fully mature elongating complexes capable of appropriate mRNA processing.
| Item Type: | Paper |
|---|---|
| Uncontrolled Keywords: | ubiquitin dependent proteolysis polymerase_II reaction carboxy terminal domain Saccharomyces Sereviciae ACTIVATE Transcription phosphorylated forms capping enzyme elongation kinase yeast DsgI Dsgl |
| Subjects: | bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > transcription |
| CSHL Authors: | |
| Communities: | CSHL labs > Tansey lab School of Biological Sciences > Publications |
| Depositing User: | CSHL Librarian |
| Date: | March 2005 |
| Date Deposited: | 09 Jan 2012 18:40 |
| Last Modified: | 19 Sep 2014 15:50 |
| Related URLs: | |
| URI: | https://repository.cshl.edu/id/eprint/22658 |
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