Losada, A., Yokochi, T., Hirano, T. (May 2005) Functional contribution of Pds5 to cohesin-mediated cohesion in human cells and Xenopus egg extracts. J Cell Sci, 118 (Pt 10). pp. 2133-41. ISSN 0021-9533 (Print)
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Abstract
Sister chromatid cohesion is essential for proper segregation of the genome in mitosis and meiosis. Central to this process is cohesin, a multi-protein complex conserved from yeast to human. Previous genetic studies in fungi have identified Pds5/BimD/Spo76 as an additional factor implicated in cohesion. Here we describe the biochemical and functional characterization of two Pds5-like proteins, Pds5A and Pds5B, from vertebrate cells. In HeLa cells, Pds5 proteins physically interact with cohesin and associate with chromatin in a cohesin-dependent manner. Depletion of the cohesin subunit Scc1 by RNA interference leads to the assembly of chromosomes with severe cohesion defects. A similar yet milder set of defects is observed in cells with reduced levels of Pds5A or Pds5B. In Xenopus egg extracts, mitotic chromosomes assembled in the absence of Pds5A and Pds5B display no discernible defects in arm cohesion, but centromeric cohesion is apparently loosened. Unexpectedly, these chromosomes retain an unusually high level of cohesin. Thus, Pds5 proteins seem to affect the stable maintenance of cohesin-mediated cohesion and its efficient dissolution during mitosis. We propose that Pds5 proteins play both positive and negative roles in sister chromatid cohesion, possibly by directly modulating the dynamic interaction of cohesin with chromatin. This idea would explain why cells lacking Pds5 function display rather complex and diverse phenotypes in different organisms.
| Item Type: | Paper |
|---|---|
| Uncontrolled Keywords: | Animals Cell Cycle Proteins metabolism Cell Extracts Centromere metabolism Chromatids metabolism Chromatin metabolism Chromosomal Proteins Non-Histone metabolism Chromosomes metabolism Female Fungal Proteins metabolism Hela Cells Humans Mitosis Nuclear Proteins metabolism Oocytes metabolism Xenopus metabolism Xenopus Proteins metabolism Pds5A and Pds5B |
| Subjects: | bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > chromatid bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > cohesin complex |
| CSHL Authors: | |
| Communities: | CSHL labs > Hirano lab |
| Depositing User: | CSHL Librarian |
| Date: | 15 May 2005 |
| Date Deposited: | 10 Jan 2012 15:34 |
| Last Modified: | 06 Nov 2017 20:08 |
| Related URLs: | |
| URI: | https://repository.cshl.edu/id/eprint/22636 |
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