Javaherian, A., Cline, H. T. (February 2005) Coordinated motor neuron axon growth and neuromuscular synaptogenesis are promoted by CPG15 in vivo. Neuron, 45 (4). pp. 505-512. ISSN 0896-6273
Abstract
We have used in vivo time-lapse two-photon imaging of single motor neuron axons labeled with GFP combined with labeling of presynaptic vesicle clusters and postsynaptic acetylcholine receptors in Xenopus laevis tadpoles to determine the dynamic rearrangement of individual axon branches and synaptogenesis during motor axon arbor development. Control GFP-labeled axons are highly dynamic during the period when axon arbors are elaborating. Axon branches emerge from sites of synaptic vesicle clusters. These data indicate that motor neuron axon elaboration and synaptogenesis are concurrent and iterative. We tested the role of Candidate Plasticity Gene 15 (CPG15, also known as Neuritin), an activity-regulated gene that is expressed in the developing motor neurons in this process. CPG15 expression enhances the development of motor neuron axon arbors by promoting neuromuscular synaptogenesis and by increasing the addition of new axon branches.
| Item Type: | Paper |
|---|---|
| Uncontrolled Keywords: | synaptic competition axon arbor hippocampal neurons neurotrophic factor retinotecial axon xenopus tadpones dendritic arbor transgenic mice NMDA recepter stop signal DYNAMICS |
| Subjects: | organs, tissues, organelles, cell types and functions > tissues types and functions > axon bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > green fluorescent protein Investigative techniques and equipment > microscopy > flourescence microscopy > two-photon excitation microscopy |
| CSHL Authors: | |
| Communities: | CSHL labs > Cline lab |
| Depositing User: | CSHL Librarian |
| Date: | February 2005 |
| Date Deposited: | 10 Jan 2012 17:05 |
| Last Modified: | 07 May 2018 15:39 |
| Related URLs: | |
| URI: | https://repository.cshl.edu/id/eprint/22609 |
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