Methods for the analysis of adenosine-to-inosine editing in RNA

Zhang, Z., Carmichael, G. G. (2004) Methods for the analysis of adenosine-to-inosine editing in RNA. In: mRNA Processing and Metabolism: methods and protocols. Methods Mol Biol, 257 . Humana Press, pp. 75-84. ISBN 1064-3745 (Print)

DOI: 10.1385/1-59259-750-5:075


In this work we describe methods for the analysis of RNAs that have been edited by the double-strand RNA-specific adenosine deaminase, ADAR. These RNAs contain inosine residues that can be detected and quantified by a variety of approaches, including base hydrolysis and thin-layer chromatography, reverse transcription polymerase chain reaction, primer extension, and inosine-specific base cleavage. The most common method for the analysis of editing will be described here. This method involves complete hydrolysis of edited RNAs to nucleoside monophosphates, followed by separation of the products using thin-layer chromatography.

Item Type: Book Section
Uncontrolled Keywords: Adenosine analysis Adenosine Deaminase metabolism Chromatography Thin Layer methods Humans Hydrolysis Inosine analysis RNA analysis metabolism RNA Editing RNA Precursors genetics RNA Messenger genetics Reverse Transcriptase Polymerase Chain Reaction/methods
Subjects: biotechnology > chromatography
CSHL Authors:
Depositing User: CSHL Librarian
Date: 2004
Date Deposited: 13 Jan 2012 20:52
Last Modified: 13 Jan 2012 20:52

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