RNAi and expression of retrotransposons MuERV-L and IAP in preimplantation mouse embryos

Svoboda, P., Stein, P., Anger, M., Bernstein, E., Hannon, G. J., Schultz, R. M. (May 2004) RNAi and expression of retrotransposons MuERV-L and IAP in preimplantation mouse embryos. Developmental Biology, 269 (1). pp. 276-285. ISSN 0012-1606

URL: https://www.ncbi.nlm.nih.gov/pubmed/15081373
DOI: 10.1016/j.ydbio.2004.01.028


Both murine endogenous retrovirus-L (MuERV-L) and intracisternal A particle (IAP), two autonomous long terminal repeat (LTR) retrotransposons, are activated during genome activation in the preimplantation mouse embryo, and both sense and antisense transcripts are detected in 2-cell and 8-cell stage embryos. Because RNA interference (RNAi) functions in the preimplantation mouse embryo, we analyzed the relationship between RNAi and MuERV-L and IAP expression by inhibiting RNAi and measuring relative changes of the levels of these transcripts. We inhibited the initial step in the RNAi pathway by injecting 1-cell embryos with mDicer siRNA or long mDicer dsRNA and analyzed MuERV-L and IAP expression at the 8-cell stage. This approach resulted in the targeted destruction of mDicer mRNA, but not Hdac1 mRNA, inhibited the RNAi pathway, and resulted in a 50% increase in IAP and MuERV-L transcript abundance. These results suggest that RNAi constrains expression of repetitive parasitic sequences in preimplantation embryos, and thereby contributes to preserving genomic integrity at a stage of development when the organism consists of only a few cells. (C) 2004 Elsevier Inc. All rights reserved.

Item Type: Paper
Uncontrolled Keywords: RNAi retrotransposon dicer preimplantation embryo genome activation YEAST SCHIZOSACCHAROMYCES-POMBE yeast Schizosaccharomyces Pombe MURINE ENDOGENOUS RETROVIRUS murine endogenous retrovirus DOUBLE-STRANDED-RNA double stranded RNA GENE-EXPRESSION gene expression C-ELEGANS C-Elegans C Elegans PATERNAL GENOME paternal genome CENTROMERIC DNA centromeric DNA INTERFERENCE inteference METHYLATION methylation ELEMENTS elements
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > RNAi
organism description > virus > retrovirus
CSHL Authors:
Communities: CSHL labs > Hannon lab
Depositing User: CSHL Librarian
Date: May 2004
Date Deposited: 25 Jan 2012 15:57
Last Modified: 02 Dec 2016 17:10
Related URLs:
URI: https://repository.cshl.edu/id/eprint/22496

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