Dynamics of single mRNPs in nuclei of living cells

Shav-Tal, Y., Darzacq, X., Shenoy, S. M., Fusco, D., Janicki, S. M., Spector, D. L., Singer, R. H. (June 2004) Dynamics of single mRNPs in nuclei of living cells. Science, 304 (5678). pp. 1797-1800. ISSN 0036-8075

URL: http://www.ncbi.nlm.nih.gov/pubmed/15205532
DOI: 10.1126/science.1099754


Understanding gene expression requires the ability to follow the fate of individual molecules. Here we use a cellular system for monitoring messenger RNA (mRNA) expression to characterize the movement in real time of single mRNA-protein complexes (mRNPs) in the nucleus of living mammalian cells. This mobility was not directed but was governed by simple diffusion. Some mRNPs were partially corralled throughout the nonhomogenous nuclear environment, but no accumulation at subnuclear domains was observed. Following energy deprivation, energy-independent motion of mRNPs was observed in a highly ATP-dependent nuclear environment; movements were constrained to chromatin-poor domains and excluded by newly formed chromatin barriers. This observation resolves a controversy, showing that the energetic requirements of nuclear mRNP trafficking are consistent with a diffusional model.

Item Type: Paper
Uncontrolled Keywords: MESSENGER-RNA messenger RNA MOVEMENT DIFFUSION diffusion MOBILITY mobility visualization VISUALIZATION PROTEINS proteins BINDING binding
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > mRNA dynamics
organism description > animal > mammal
CSHL Authors:
Communities: CSHL labs > Spector lab
Depositing User: CSHL Librarian
Date: June 2004
Date Deposited: 26 Jan 2012 15:01
Last Modified: 29 Jan 2015 15:18
Related URLs:
URI: https://repository.cshl.edu/id/eprint/22478

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