Proteomic analysis of interchromatin granule clusters

Saitoh, N., Spahr, C. S., Patterson, S. D., Bubulya, P., Neuwald, A. F., Spector, D. L. (August 2004) Proteomic analysis of interchromatin granule clusters. Mol Biol Cell, 15 (8). pp. 3876-90. ISSN 1059-1524 (Print)

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DOI: 10.1091/mbc.E04-03-0253


A variety of proteins involved in gene expression have been localized within mammalian cell nuclei in a speckled distribution that predominantly corresponds to interchromatin granule clusters (IGCs). We have applied a mass spectrometry strategy to identify the protein composition of this nuclear organelle purified from mouse liver nuclei. Using this approach, we have identified 146 proteins, many of which had already been shown to be localized to IGCs, or their functions are common to other already identified IGC proteins. In addition, we identified 32 proteins for which only sequence information is available and thus these represent novel IGC protein candidates. We find that 54% of the identified IGC proteins have known functions in pre-mRNA splicing. In combination with proteins involved in other steps of pre-mRNA processing, 81% of the identified IGC proteins are associated with RNA metabolism. In addition, proteins involved in transcription, as well as several other cellular functions, have been identified in the IGC fraction. However, the predominance of pre-mRNA processing factors supports the proposed role of IGCs as assembly, modification, and/or storage sites for proteins involved in pre-mRNA processing.

Item Type: Paper
Uncontrolled Keywords: Animals Cell Nucleus immunology metabolism Chromatin chemistry metabolism Mass Spectrometry Mice Nuclear Proteins analysis genetics physiology Protein Structure Tertiary Proteomics RNA Precursors genetics metabolism RNA Splicing RNA Messenger metabolism
Subjects: bioinformatics > genomics and proteomics > annotation > gene expression profiling annotation
CSHL Authors:
Communities: CSHL labs > Spector lab
Depositing User: CSHL Librarian
Date: August 2004
Date Deposited: 25 Jan 2012 19:48
Last Modified: 29 Jan 2015 14:59
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