Spatial and temporal regulation of Condensins I and II in mitotic chromosome assembly in human cells

Ono, T., Fang, Y., Spector, D. L., Hirano, T. (July 2004) Spatial and temporal regulation of Condensins I and II in mitotic chromosome assembly in human cells. Mol Biol Cell, 15 (7). pp. 3296-308.

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DOI: 10.1091/mbc.E04-03-0242


Two different condensin complexes make distinct contributions to metaphase chromosome architecture in vertebrate cells. We show here that the spatial and temporal distributions of condensins I and II are differentially regulated during the cell cycle in HeLa cells. Condensin II is predominantly nuclear during interphase and contributes to early stages of chromosome assembly in prophase. In contrast, condensin I is sequestered in the cytoplasm from interphase through prophase and gains access to chromosomes only after the nuclear envelope breaks down in prometaphase. The two complexes alternate along the axis of metaphase chromatids, but they are arranged into a unique geometry at the centromere/kinetochore region, with condensin II enriched near the inner kinetochore plate. This region-specific distribution of condensins I and II is severely disrupted upon depletion of Aurora B, although their association with the chromosome arm is not. Depletion of condensin subunits causes defects in kinetochore structure and function, leading to aberrant chromosome alignment and segregation. Our results suggest that the two condensin complexes act sequentially to initiate the assembly of mitotic chromosomes and that their specialized distribution at the centromere/kinetochore region may play a crucial role in placing sister kinetochores into the back-to-back orientation.

Item Type: Paper
Uncontrolled Keywords: Adenosine Triphosphatases analysis/genetics/metabolism Carrier Proteins analysis Chromosome Segregation drug effects HeLa Chromosomes Human chemistry immunology metabolism DNA-Binding Proteins analysis genetics metabolism Hela Cells Humans Intracellular Space chemistry immunology metabolism Kinetochores chemistry immunology metabolism Mitosis Multiprotein Complexes Nuclear Proteins analysis Phosphorylation Protein-Serine-Threonine Kinases metabolism RNA Interference RNA, Small Interfering pharmacology
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > condensin complex
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > metaphase
CSHL Authors:
Communities: CSHL labs > Spector lab
Depositing User: CSHL Librarian
Date: July 2004
Date Deposited: 26 Jan 2012 16:27
Last Modified: 29 Jan 2015 15:14
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