Role of p63 and basal cells in the prostate

Kurita, T., Medina, R. T., Mills, A. A., Cunha, G. R. (October 2004) Role of p63 and basal cells in the prostate. Development, 131 (20). pp. 4955-4964. ISSN 0950-1991

DOI: 10.1242/dev.0138


The prostate contains two major epithelial cell types luminal and basal cells - both of which develop from urogenital sinus epithelium. The cell linage relationship between these two epithelial types is not clear. Here we demonstrate that luminal cells can develop independently of basal cells, but that basal cells are essential for maintaining ductal integrity and the proper differentiation of luminal cells. Urogenital sinus (UGS) isolated from p63(+/+) and p63(-/-) embryos developed into prostate when grafted into adult male nude mice. Prostatic tissue that developed in p63(-/-) UGS grafts contained neuroendocrine and luminal cells, but basal cells were absent. Therefore, p63 is essential for differentiation of basal cells, but p63 and thus basal cells are not required for differentiation of prostatic neuroendocrine and luminal epithelial cells.p63(-/-) prostatic grafts also contained atypical mucinous; cells, which appeared to differentiate from luminal cells via activation of Src. In the response to castration, regression of p63(-/-) prostate was inordinately severe with almost complete loss of ducts, resulting in the formation of residual cystic structures devoid of epithelium. Therefore, basal cells play critical roles in maintaining ductal integrity and survival of luminal cells. However, regressed p63(-/-) prostate did regenerate in response to androgen administration, indicating that basal cells were not essential for prostatic regeneration.

Item Type: Paper
Uncontrolled Keywords: androgen mucin epithelial differentiation apoptosis urogenital sinus cell linage MAPK Src EPITHELIAL STEM-CELLS epithelial stem cells NF-KAPPA-B NF-Kappa-B UROTHELIAL DIFFERENTIATION urothelial differentiation GENE-EXPRESSION gene expression P53 HOMOLOG P53 homolog CANCER cancer FAMILY family KINASE kinase TISSUE tissue MUCIN mucin
Subjects: organs, tissues, organelles, cell types and functions > organs types and functions > prostate
CSHL Authors:
Communities: CSHL labs > Mills lab
Depositing User: CSHL Librarian
Date: October 2004
Date Deposited: 31 Jan 2012 17:10
Last Modified: 02 Feb 2017 20:01
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