Hu, P., Samudre, K., Wu, S., Sun, Y., Hernandez, N. (October 2004) CK2 phosphorylation of Bdp1 executes cell cycle-specific RNA polymerase III transcription repression. Mol Cell, 16 (1). pp. 81-92. ISSN 1097-2765 (Print)
Abstract
RNA polymerase III (pol III) transcription from the human U6 snRNA promoter can be reconstituted with the recombinant factors SNAPc and Brf2-TFIIIB combined with purified pol III. In this system, CK2 treatment of the pol III complex is required for transcription, whereas treatment of Brf2-TFIIIB is inhibitory. Here we show that CK2 inhibits Brf2-TFIIIB by specifically phosphorylating its Bdp1 component. Bdp1 is phosphorylated by CK2 during mitosis, and this is accompanied by Bdp1 dissociation from the U6 promoter and from chromatin in general and by transcription repression. Remarkably, whereas inhibition of CK2 in mitotic extracts restores pol III transcription, inhibition of CK2 in active S phase extracts debilitates transcription. Thus, CK2 is directed to phosphorylate different targets within the basal pol III transcription machinery at different times during the cell cycle, with opposite transcriptional effects.
| Item Type: | Paper |
|---|---|
| Uncontrolled Keywords: | Casein Kinase II Cell Cycle physiology Chromatin physiology Conserved Sequence Gene Expression Regulation physiology Humans Mitosis physiology Mutation Nuclear Proteins genetics metabolism Phosphorylation Promoter Regions (Genetics) Protein Structure Tertiary Protein Serine Threonine Kinases metabolism RNA Polymerase III metabolism Transcription Factor TFIIIB Transcription Genetic |
| Subjects: | bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > transcription bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > enzymes > RNA polymerase |
| CSHL Authors: | |
| Depositing User: | CSHL Librarian |
| Date: | 8 October 2004 |
| Date Deposited: | 03 Feb 2012 15:09 |
| Last Modified: | 03 Feb 2012 15:09 |
| URI: | https://repository.cshl.edu/id/eprint/22393 |
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