Hirano, M., Hirano, T. (July 2004) Positive and negative regulation of SMC-DNA interactions by ATP and accessory proteins. EMBO J, 23 (13). pp. 2664-73. ISSN 0261-4189 (Print)
Abstract
Structural maintenance of chromosomes (SMC) proteins are central regulators of higher-order chromosome dynamics from bacteria to humans. The Bacillus subtilis SMC (BsSMC) homodimer adopts a V-shaped structure with an ATP-binding catalytic domain at each end. We report here that two small proteins, ScpA and ScpB, associate with the catalytic domains of BsSMC in an ordered fashion and suppress its ATPase activity. When combined with a 'transition state' mutant of BsSMC that poorly hydrolyzes ATP, ScpA promotes stable engagement of two catalytic domains in an ATP-dependent manner. In solution, this occurs intramolecularly and closes the DNA-entry gate of an SMC dimer. ScpB further stabilizes this conformation and prevents BsSMC from binding to double-stranded DNA (dsDNA). In contrast, when the mutant BsSMC is first allowed to interact with dsDNA, subsequent addition of ScpA leads to assembly of large nucleoprotein complexes, possibly by stabilizing intermolecular engagement of the catalytic domains from different SMC dimers. We propose that the ATP-modulated engagement/disengagement cycle of SMC proteins plays both positive and negative roles in their dynamic interactions with dsDNA.
| Item Type: | Paper |
|---|---|
| Uncontrolled Keywords: | Adenosine Triphosphatases chemistry metabolism Bacillus subtilis metabolism Bacterial Proteins chemistry genetics isolation & purification metabolism Catalytic Domain Centrifugation Density Gradient Chromosomes Bacterial metabolism DNA Bacterial metabolism Dimerization Models Biological Mutation Precipitin Tests Protein Conformation |
| Subjects: | bioinformatics > genomics and proteomics > small molecules > ATP bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > chromosome bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > chromosomes, structure and function > chromosome |
| CSHL Authors: | |
| Depositing User: | CSHL Librarian |
| Date: | 7 July 2004 |
| Date Deposited: | 01 Feb 2012 18:45 |
| Last Modified: | 01 Feb 2012 18:45 |
| URI: | https://repository.cshl.edu/id/eprint/22388 |
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