The two cytochrome c species, DC3 and DC4, are not required for caspase activation and apoptosis in Drosophila cells

Dorstyn, L., Mills, K., Lazebnik, Y., Kumar, S. (November 2004) The two cytochrome c species, DC3 and DC4, are not required for caspase activation and apoptosis in Drosophila cells. Journal of Cell Biology, 167 (3). pp. 405-410. ISSN 0021-9525

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In Drosophila, activation of the apical caspase DRONC requires the apoptotic protease-activating factor homologue, DARK. However, unlike caspase activation in mammals, DRONC activation is not accompanied by the release of cytochrome c from mitochondria. Drosophila encodes two cytochrome c proteins, Cytc-p (DC4) the predominantly expressed species, and Cytc-d (DC3), which is implicated in caspase activation during spermatogenesis. Here, we report that silencing expression of either or both DC3 and DC4 had no effect on apoptosis or activation of DRONC and DRICE in Drosophila cells. We find that loss of function mutations in dc3 and dc4, do not affect caspase activation during Drosophila development and that ectopic expression of DC3 or DC4 in Drosophila cells does not induce caspase activation. in cell-free studies, recombinant DC3 or DC4 failed to activate caspases in Drosophila cell lysates, but remarkably induced caspase activation in extracts from human cells. Overall, our results argue that DARK-mediated DRONC activation occurs independently of cytochrome c.

Item Type: Paper
Uncontrolled Keywords: DEATH MACHINERY death machinery MELANOGASTER Melanogaster HOMOLOG homolog COMPLEX complex DRONC EXPRESSION expression pathway PATHWAY APAF-1
Subjects: organism description > animal > insect > Drosophila
organs, tissues, organelles, cell types and functions > cell types and functions > cell functions > apoptosis
CSHL Authors:
Communities: CSHL labs > Labeznik lab
Depositing User: CSHL Librarian
Date: November 2004
Date Deposited: 03 Feb 2012 16:00
Last Modified: 03 Feb 2012 16:00
PMCID: PMC2172470

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