Ren, J., Wang, C. L., Sternglanz, R. (October 2010) Promoter strength influences the S phase requirement for establishment of silencing at the Saccharomyces cerevisiae silent mating type loci. Genetics, 186 (2). pp. 551-560.
Abstract
In Saccharomyces cerevisiae, the two cryptic mating type loci, HML and HMR, are transcriptionally silent. Previous studies on the establishment of silencing at HMR identified a requirement for passage through S phase. However, the underlying mechanism for this requirement is still unknown. In contrast to HMR, we found that substantial silencing of HML could be established without passage through S phase. To understand this difference, we analyzed several chimeric HM loci and found that promoter strength determined the S phase requirement. To silence a locus with a strong promoter such as the a1/a2 promoter required passage through S phase while HM loci with weaker promoters such as the α1/α2 or TRP1 promoter did not show this requirement. Thus, transcriptional activity counteracts the establishment of silencing but can be overcome by passage through S phase. Copyright © 2010 by the Genetics Society of America.
| Item Type: | Paper |
|---|---|
| Subjects: | bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > transcription organism description > yeast > Saccharomyces |
| CSHL Authors: | |
| Communities: | CSHL Post Doctoral Fellows CSHL labs > Martienssen lab CSHL labs > Van Aelst lab |
| Depositing User: | CSHL Librarian |
| Date: | October 2010 |
| Date Deposited: | 04 Oct 2011 15:22 |
| Last Modified: | 03 May 2013 14:07 |
| PMCID: | PMC2954484 |
| Related URLs: | |
| URI: | https://repository.cshl.edu/id/eprint/15513 |
Actions (login required)
 |
Administrator's edit/view item |