Nagura, H., Irie, K., Imai, T., Shimomura, T., Hige, T., Fujiyoshi, Y. (August 2010) Evidence for lateral mobility of voltage sensors in prokaryotic voltage-gated sodium channels. Biochemical and Biophysical Research Communications, 399 (3). pp. 341-346.
Abstract
Voltage-sensor domains (VSDs) in voltage-gated ion channels are thought to regulate the probability that a channel adopts an open conformation by moving vertically in the lipid bilayer. Here we characterized the movement of the VSDs of the prokaryotic voltage-gated sodium channel, NaChBac. Substitution of residue T110, which is located on the extracellular side of the fourth transmembrane helix of the VSD, by cysteine resulted in the formation of a disulfide bond between adjacent subunits in the channel. Our results suggest that T110 residues in VSDs of adjacent subunits can come into close proximity, implying that the VSDs can move laterally in the membrane and constitute a mechanism that regulates channel activity. © 2010 Elsevier Inc. All rights reserved.
| Item Type: | Paper |
|---|---|
| Uncontrolled Keywords: | Disulfide bond formation Lateral movement NaChBac S4 segment Tandem tetramer Voltage sensor |
| Subjects: | organs, tissues, organelles, cell types and functions > tissues types and functions > membranes |
| CSHL Authors: | |
| Communities: | CSHL Post Doctoral Fellows CSHL labs > Turner lab |
| Depositing User: | CSHL Librarian |
| Date: | 27 August 2010 |
| Date Deposited: | 05 Oct 2011 15:04 |
| Last Modified: | 02 May 2013 15:55 |
| Related URLs: | |
| URI: | https://repository.cshl.edu/id/eprint/15492 |
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