A novel role for 14-3-3σ in regulating epithelial cell polarity

Ling, C., Zuo, D., Xue, B., Muthuswamy, S. K., Muller, W. J. (2010) A novel role for 14-3-3σ in regulating epithelial cell polarity. Genes and Development, 24 (9). pp. 947-956.

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URL: https://www.ncbi.nlm.nih.gov/pubmed/20439433
DOI: 10.1101/gad.1896810


The loss of epithelial polarity is thought to play an important role during mammary tumor progression. Using a unique transgenic mouse model of ErbB2-induced mammary tumorigenesis, we demonstrated previously that amplification of ErbB2 is frequently accompanied by loss of the 14-3-3σ gene. Here, we demonstrate that ectopic expression of 14-3-3σ results in restoration of epithelial polarity in ErbB2-transformed mammary tumor cells. We further demonstrate that targeted deletion of 14-3-3σ within primary mammary epithelial cells increases their proliferative capacity and adversely affects their ability to form polarized structures. Finally, we show that 14-3-3σ can specifically form complexes with Par3, a protein that is essential for the maintenance of a polarized epithelial state. Taken together, these observations suggest that 14-3-3σ plays a critical role in retaining epithelial polarity. © 2010 by Cold Spring Harbor Laboratory Press.

Item Type: Paper
Uncontrolled Keywords: 14-3-3σ Conditional knockout Epithelial polarity ErbB2
Subjects: diseases & disorders > cancer > cancer types > breast cancer
organism description > animal > mammal > rodent > mouse
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > transgenic animal
CSHL Authors:
Communities: CSHL labs > Muthuswamy lab
Depositing User: CSHL Librarian
Date: 2010
Date Deposited: 03 Oct 2011 20:47
Last Modified: 08 Mar 2018 19:54
PMCID: PMC2861193
Related URLs:
URI: https://repository.cshl.edu/id/eprint/15464

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