Ling, C., Zuo, D., Xue, B., Muthuswamy, S. K., Muller, W. J. (2010) A novel role for 14-3-3σ in regulating epithelial cell polarity. Genes and Development, 24 (9). pp. 947-956.
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Abstract
The loss of epithelial polarity is thought to play an important role during mammary tumor progression. Using a unique transgenic mouse model of ErbB2-induced mammary tumorigenesis, we demonstrated previously that amplification of ErbB2 is frequently accompanied by loss of the 14-3-3σ gene. Here, we demonstrate that ectopic expression of 14-3-3σ results in restoration of epithelial polarity in ErbB2-transformed mammary tumor cells. We further demonstrate that targeted deletion of 14-3-3σ within primary mammary epithelial cells increases their proliferative capacity and adversely affects their ability to form polarized structures. Finally, we show that 14-3-3σ can specifically form complexes with Par3, a protein that is essential for the maintenance of a polarized epithelial state. Taken together, these observations suggest that 14-3-3σ plays a critical role in retaining epithelial polarity. © 2010 by Cold Spring Harbor Laboratory Press.
| Item Type: | Paper |
|---|---|
| Uncontrolled Keywords: | 14-3-3σ Conditional knockout Epithelial polarity ErbB2 |
| Subjects: | diseases & disorders > cancer > cancer types > breast cancer organism description > animal > mammal > rodent > mouse bioinformatics > genomics and proteomics > genetics & nucleic acid processing > transgenic animal |
| CSHL Authors: | |
| Communities: | CSHL labs > Muthuswamy lab |
| Depositing User: | CSHL Librarian |
| Date: | 2010 |
| Date Deposited: | 03 Oct 2011 20:47 |
| Last Modified: | 08 Mar 2018 19:54 |
| PMCID: | PMC2861193 |
| Related URLs: | |
| URI: | https://repository.cshl.edu/id/eprint/15464 |
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