Gottesman, A., Milazzo, J., Lazebnik, Y. (October 2010) V-fusion: a convenient, nontoxic method for cell fusion. Biotechniques, 49 (4). pp. 747-50. ISSN 1940-9818 (Electronic) 0736-6205 (Linking)
Abstract
Cell-to-cell fusion (cell fusion) is a fundamental biological process that also has been used as a versatile experimental tool to dissect a variety of cellular mechanisms, including the consequences of cell fusion itself, and to produce cells with desired properties, such as hybridomas and reprogrammed progenitors. However, current methods of cell fusion are not satisfactory because of their toxicity, inefficiency, or lack of flexibility. We describe a simple, versatile, scalable, and nontoxic approach that we call V-fusion, as it is based on the ability of the vesicular stomatitis virus G protein (VSV-G), a viral fusogen of broad tropism, to become rapidly and reversibly activated. We suggest that this approach will benefit a broad array of studies that investigate consequences of cell fusion or use cell fusion as an experimental tool. Address correspondence to Yuri Lazebnik, Cold Spring Harbor Laboratory, Cold Spring Harbor, NY 11768, USA. e-mail:
| Item Type: | Paper |
|---|---|
| Subjects: | Investigative techniques and equipment > cell fusion organism description > virus |
| CSHL Authors: | |
| Communities: | CSHL labs > Labeznik lab |
| Depositing User: | CSHL Librarian |
| Date: | October 2010 |
| Date Deposited: | 30 Sep 2011 13:34 |
| Last Modified: | 02 Mar 2018 19:58 |
| Related URLs: | |
| URI: | https://repository.cshl.edu/id/eprint/15423 |
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