Fu, Y., Huang, Z. J. (December 2010) Differential dynamics and activity-dependent regulation of alpha- and beta-neurexins at developing GABAergic synapses. Proceedings of the National Academy of Sciences of the United States of America, 107 (52). pp. 22699-704. ISSN 0027-8424
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Abstract
Neurexins (NRXs) and neuroligins are key synaptic adhesion molecules that also recruit synaptic signaling machineries. Neurexins consist of alpha- and beta-isoforms, but how they couple synaptic transmission and adhesion to regulate activity-dependent synapse development remains unclear, in part because of poor understanding of their cell biology and regulation in the relevant neurons. Here, we examined the subaxonal localization, dynamics, and regulation of NRX1alpha and NRX1beta in cortical perisomatic inhibitory synapses. Both isoforms are delivered to presynaptic terminals but show significant and different turnover rate at the membrane. Although NRX1alpha is highly diffuse along developing axons and filopodia, NRX1beta is strictly anchored at terminals through binding to postsynaptic ligands. The turnover rate of NRX1beta is attenuated by neural activity and presynaptic GABA(B) receptors. NRXs, thus, are intrinsically dynamic but are stabilized by local transmitter release. Such an activity-adjusted adhesion system seems ideally suited to rapidly explore and validate synaptic partners guided by synaptic transmission.
| Item Type: | Paper |
|---|---|
| Subjects: | bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > neurexin |
| CSHL Authors: | |
| Communities: | CSHL labs > Huang lab |
| Depositing User: | CSHL Librarian |
| Date: | 28 December 2010 |
| Date Deposited: | 30 Sep 2011 15:29 |
| Last Modified: | 05 Jan 2018 17:25 |
| PMCID: | PMC3012487 |
| Related URLs: | |
| URI: | https://repository.cshl.edu/id/eprint/15414 |
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