Fischer, Kyle B, Collins, Hannah K, Pang, Yan, Roy, Dheeraj S, Zhang, Ying, Feng, Guoping, Li, Shu-Jing, Kepecs, Adam, Callaway, Edward M (January 2023) Monosynaptic restriction of the anterograde herpes simplex virus strain H129 for neural circuit tracing. Journal of Comparative Neurology. ISSN 0021-9967
Abstract
Identification of synaptic partners is a fundamental task for systems neuroscience. To date, few reliable techniques exist for whole brain labeling of downstream synaptic partners in a cell-type-dependent and monosynaptic manner. Herein, we describe a novel monosynaptic anterograde tracing system based on the deletion of the gene UL6 from the genome of a cre-dependent version of the anterograde Herpes Simplex Virus 1 strain H129. Given that this knockout blocks viral genome packaging and thus viral spread, we reasoned that co-infection of a HSV H129 ΔUL6 virus with a recombinant adeno-associated virus expressing UL6 in a cre-dependent manner would result in monosynaptic spread from target cre-expressing neuronal populations. Application of this system to five nonreciprocal neural circuits resulted in labeling of neurons in expected projection areas. While some caveats may preclude certain applications, this system provides a reliable method to label postsynaptic partners in a brain-wide fashion.
| Item Type: | Paper |
|---|---|
| Subjects: | bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > chromosomal deletions organism description > virus > herpes simplex virus organs, tissues, organelles, cell types and functions > cell types and functions > cell functions > synaptic transmission |
| CSHL Authors: | |
| Communities: | CSHL labs > Kepecs lab |
| SWORD Depositor: | CSHL Elements |
| Depositing User: | CSHL Elements |
| Date: | 6 January 2023 |
| Date Deposited: | 30 Jan 2023 15:53 |
| Last Modified: | 30 Jan 2023 15:53 |
| URI: | https://repository.cshl.edu/id/eprint/40803 |
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