Monosynaptic restriction of the anterograde herpes simplex virus strain H129 for neural circuit tracing

Fischer, Kyle B, Collins, Hannah K, Pang, Yan, Roy, Dheeraj S, Zhang, Ying, Feng, Guoping, Li, Shu-Jing, Kepecs, Adam, Callaway, Edward M (January 2023) Monosynaptic restriction of the anterograde herpes simplex virus strain H129 for neural circuit tracing. Journal of Comparative Neurology. ISSN 0021-9967

DOI: 10.1002/cne.25451


Identification of synaptic partners is a fundamental task for systems neuroscience. To date, few reliable techniques exist for whole brain labeling of downstream synaptic partners in a cell-type-dependent and monosynaptic manner. Herein, we describe a novel monosynaptic anterograde tracing system based on the deletion of the gene UL6 from the genome of a cre-dependent version of the anterograde Herpes Simplex Virus 1 strain H129. Given that this knockout blocks viral genome packaging and thus viral spread, we reasoned that co-infection of a HSV H129 ΔUL6 virus with a recombinant adeno-associated virus expressing UL6 in a cre-dependent manner would result in monosynaptic spread from target cre-expressing neuronal populations. Application of this system to five nonreciprocal neural circuits resulted in labeling of neurons in expected projection areas. While some caveats may preclude certain applications, this system provides a reliable method to label postsynaptic partners in a brain-wide fashion.

Item Type: Paper
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > chromosomal deletions
organism description > virus > herpes simplex virus
organs, tissues, organelles, cell types and functions > cell types and functions > cell functions > synaptic transmission
CSHL Authors:
Communities: CSHL labs > Kepecs lab
SWORD Depositor: CSHL Elements
Depositing User: CSHL Elements
Date: 6 January 2023
Date Deposited: 30 Jan 2023 15:53
Last Modified: 30 Jan 2023 15:53

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