Measuring Circadian Neutrophil Infiltration in Tissues by Paired Whole-Mount Tissue Clearing and Flow Cytometry

Vicanolo, Tommaso, Hidalgo, Andres, Adrover, Jose M (May 2022) Measuring Circadian Neutrophil Infiltration in Tissues by Paired Whole-Mount Tissue Clearing and Flow Cytometry. In: Methods in Molecular Biology. Springer US, pp. 265-284. ISBN 9781071622483

Abstract

Neutrophils infiltrate most tissues in the organism in the steady state, often following circadian patterns. Neutrophil infiltration is also key to immune defense under inflammatory conditions. In all cases, accurate measurements of the absolute number of infiltrated cells and of their localization are important to understand steady-state or inflammatory migration patterns and kinetics. Here we present a method to obtain accurate information on both neutrophil number and distribution that can be successfully applied to circadian studies of neutrophil (or any other cell of interest) migration in vivo. Moreover, this method can be also used to obtain information on activation states or effector functions, for example, by measurement of neutrophil extracellular trap formation in tissues.

Item Type:	Book Section
Subjects:	Investigative techniques and equipment organs, tissues, organelles, cell types and functions > cell types and functions > cell functions > cell cycle > Neutrophil extracellular traps organs, tissues, organelles, cell types and functions > cell types and functions > cell functions > cell cycle organs, tissues, organelles, cell types and functions > cell types and functions > cell functions organs, tissues, organelles, cell types and functions > cell types and functions Investigative techniques and equipment > flow cytometry organs, tissues, organelles, cell types and functions > cell types and functions > cell types > neutrophils organs, tissues, organelles, cell types and functions > cell types and functions > cell types > neutrophils organs, tissues, organelles, cell types and functions > cell types and functions > cell types > neutrophils organs, tissues, organelles, cell types and functions
CSHL Authors:	Adrover, Jose M.
Communities:	CSHL labs > Egeblad lab
SWORD Depositor:	CSHL Elements
Depositing User:	CSHL Elements
Date:	25 May 2022
Date Deposited:	08 Jun 2022 19:49
Last Modified:	17 Jan 2024 15:53
URI:	https://repository.cshl.edu/id/eprint/40654

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