In Vivo Imaging of Circadian NET Formation During Lung Injury by Four-Dimensional Intravital Microscopy

Aroca-Crevillén, Alejandra, Hidalgo, Andres, Adrover, Jose M (May 2022) In Vivo Imaging of Circadian NET Formation During Lung Injury by Four-Dimensional Intravital Microscopy. In: Methods in Molecular Biology. Springer US, pp. 285-300. ISBN 9781071622483

Abstract

Neutrophil extracellular traps (NETs) are toxic extracellular structures deployed by neutrophils in response to pathogens and sterile danger signals. NETs are circadian in nature as mouse and human neutrophils preferentially deploy them at night or early morning. Traditionally, NETs have been quantified using a plethora of methods including immunofluorescence and ELISA-based assays; however few options are available to visualize them in vivo. Here we describe a method to directly visualize and quantify NET formation and release in the microvasculature of the lung using intravital imaging in a model of acute lung injury. The method allows four-dimensional capture and quantification of NET formation dynamics over time and should be a useful resource for those interested in visualizing neutrophil responses in vivo.

Item Type:	Book Section
Subjects:	Investigative techniques and equipment organs, tissues, organelles, cell types and functions > cell types and functions > cell functions > cell cycle > Neutrophil extracellular traps organism description > animal organs, tissues, organelles, cell types and functions > cell types and functions > cell functions > cell cycle organs, tissues, organelles, cell types and functions > cell types and functions > cell functions organs, tissues, organelles, cell types and functions > cell types and functions Investigative techniques and equipment > imaging Investigative techniques and equipment > imaging > intravital imaging organs, tissues, organelles, cell types and functions > organs types and functions > lung organism description > animal > mammal organism description > animal > mammal > rodent > mouse organs, tissues, organelles, cell types and functions > organs types and functions organs, tissues, organelles, cell types and functions organism description > animal > mammal > rodent
CSHL Authors:	Adrover, Jose M.
Communities:	CSHL labs > Egeblad lab
SWORD Depositor:	CSHL Elements
Depositing User:	CSHL Elements
Date:	25 May 2022
Date Deposited:	08 Jun 2022 19:42
Last Modified:	11 Jan 2024 15:37
URI:	https://repository.cshl.edu/id/eprint/40653

Actions (login required)

Administrator's edit/view item