Antibody cocktail to SARS-CoV-2 spike protein prevents rapid mutational escape seen with individual antibodies.

Baum, Alina, Fulton, Benjamin O, Wloga, Elzbieta, Copin, Richard, Pascal, Kristen E, Russo, Vincenzo, Giordano, Stephanie, Lanza, Kathryn, Negron, Nicole, Ni, Min, Wei, Yi, Atwal, Gurinder S, Murphy, Andrew J, Stahl, Neil, Yancopoulos, George D, Kyratsous, Christos A (August 2020) Antibody cocktail to SARS-CoV-2 spike protein prevents rapid mutational escape seen with individual antibodies. Science, 369 (6506). pp. 1014-1018. ISSN 0036-8075

URL: https://www.ncbi.nlm.nih.gov/pubmed/32540904
DOI: 10.1126/science.abd0831

Abstract

Antibodies targeting the spike protein of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) present a promising approach to combat the coronavirus disease 2019 (COVID-19) pandemic; however, concerns remain that mutations can yield antibody resistance. We investigated the development of resistance against four antibodies to the spike protein that potently neutralize SARS-CoV-2, individually as well as when combined into cocktails. These antibodies remain effective against spike variants that have arisen in the human population. However, novel spike mutants rapidly appeared after in vitro passaging in the presence of individual antibodies, resulting in loss of neutralization; such escape also occurred with combinations of antibodies binding diverse but overlapping regions of the spike protein. Escape mutants were not generated after treatment with a noncompeting antibody cocktail.

Item Type: Paper
Subjects: diseases & disorders > viral diseases > coronavirus > therapies and treatments > antibody therapy
diseases & disorders > viral diseases > coronavirus > covid 19
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > mutations
CSHL Authors:
Communities: CSHL labs > Atwal lab
SWORD Depositor: CSHL Elements
Depositing User: CSHL Elements
Date: 21 August 2020
Date Deposited: 07 May 2021 13:08
Last Modified: 07 May 2021 13:08
PMCID: PMC7299283
URI: https://repository.cshl.edu/id/eprint/40036

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