Podoplanin, a Potential Therapeutic Target for Nasopharyngeal Carcinoma

Hsu, Y. B., Huang, C. F., Lin, K. T., Kuo, Y. L., Lan, M. C., Lan, M. Y. (June 2019) Podoplanin, a Potential Therapeutic Target for Nasopharyngeal Carcinoma. Biomed Res Int, 2019. p. 7457013.

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URL: https://www.ncbi.nlm.nih.gov/pubmed/31321241
DOI: 10.1155/2019/7457013

Abstract

Introduction: The role of podoplanin (PDPN) in nasopharyngeal carcinoma (NPC) is still unknown. The aims of this study were to investigate the expression and role of PDPN in NPC cells. Materials and Methods: Immunofluorescence staining and functional tests were used to determine the effects of PDPN knockdown by siRNA in TW01 NPC cells. Microarray analysis was conducted to identify genes regulated by PDPN. The molecular mechanism of PDPN on NPC cells was further determined by Ingenuity Pathways Analysis (IPA). Results: PDPN was expressed in most TW01 NPC cells. PDPN knockdown by siRNA decreased NPC cell proliferation, migration, and invasion. The microarray data showed 63 upregulated genes and 12 downregulated genes following PDPN knockdown. The top 5 most upregulated genes analyzed by IPA were IFI27, IFI44L, IFI6, OAS1, and TRIM22, and the most relevant pathway was the interferon signaling pathway. Conclusions: To the best of our knowledge, this is the first report to show that knocking down PDPN leads to suppression of NPC cell proliferation, migration, and invasion. Our results suggest that PDPN may serve as a potential chemotherapeutic target for NPC treatment in the future.

Item Type: Paper
Subjects: diseases & disorders > cancer
bioinformatics > genomics and proteomics > analysis and processing > microarray gene expression processing
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > siRNA
CSHL Authors:
Communities: CSHL labs > Krainer lab
Depositing User: Matthew Dunn
Date: 20 June 2019
Date Deposited: 09 Aug 2019 15:16
Last Modified: 09 Aug 2019 15:16
PMCID: PMC6610758
Related URLs:
URI: https://repository.cshl.edu/id/eprint/38218

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