Illuminating spatial A-to-I RNA editing signatures within the Drosophila brain

Sapiro, A. L., Shmueli, A., Henry, G. L., Li, Q., Shalit, T., Yaron, O., Paas, Y., Billy Li, J., Shohat-Ophir, G. (February 2019) Illuminating spatial A-to-I RNA editing signatures within the Drosophila brain. Proc Natl Acad Sci U S A, 116 (6). pp. 2318-2327. ISSN 0027-8424

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Abstract

Adenosine-to-inosine (A-to-I) RNA editing, catalyzed by ADAR enzymes, is a ubiquitous mechanism that generates transcriptomic diversity. This process is particularly important for proper neuronal function; however, little is known about how RNA editing is dynamically regulated between the many functionally distinct neuronal populations of the brain. Here, we present a spatial RNA editing map in the Drosophila brain and show that different neuronal populations possess distinct RNA editing signatures. After purifying and sequencing RNA from genetically marked groups of neuronal nuclei, we identified a large number of editing sites and compared editing levels in hundreds of transcripts across nine functionally different neuronal populations. We found distinct editing repertoires for each population, including sites in repeat regions of the transcriptome and differential editing in highly conserved and likely functional regions of transcripts that encode essential neuronal genes. These changes are site-specific and not driven by changes in Adar expression, suggesting a complex, targeted regulation of editing levels in key transcripts. This fine-tuning of the transcriptome between different neurons by RNA editing may account for functional differences between distinct populations in the brain.

Item Type:	Paper
Subjects:	organism description > animal > insect > Drosophila organs, tissues, organelles, cell types and functions > cell types and functions > cell types > neurons organs, tissues, organelles, cell types and functions > cell types and functions > cell types > neurons organs, tissues, organelles, cell types and functions > cell types and functions > cell types > neurons bioinformatics > genomics and proteomics > genetics & nucleic acid processing > transcriptomes
CSHL Authors:	Henry, Gilbert
Communities:	CSHL labs > Zador lab
Depositing User:	Matthew Dunn
Date:	5 February 2019
Date Deposited:	15 Feb 2019 19:08
Last Modified:	29 Jun 2021 20:12
PMCID:	PMC6369821
Related URLs:	Publisher
URI:	https://repository.cshl.edu/id/eprint/37699

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