Single-Cell Applications of Next-Generation Sequencing

Anaparthy, N., Ho, Y. J., Martelotto, L., Hammell, M., Hicks, J. (January 2019) Single-Cell Applications of Next-Generation Sequencing. Cold Spring Harb Perspect Med, 9 (10). ISSN 2157-1422

DOI: 10.1101/cshperspect.a026898


The single cell is considered the basic unit of biology, and the pursuit of understanding how heterogeneous populations of cells can functionally coexist in tissues, organisms, microbial ecosystems, and even cancer, makes them the subject of intense study. Next-generation sequencing (NGS) of RNA and DNA has opened a new frontier of (single)-cell biology. Hundreds to millions of cells now can be assayed in parallel, providing the molecular profile of each cell in its milieu inexpensively and in a manner that can be analyzed mathematically. The goal of this article is to provide a high-level overview of single-cell sequencing for the nonexpert and show how its applications are influencing both basic and applied clinical studies in embryology, developmental genetics, and cancer.

Item Type: Paper
Subjects: Investigative techniques and equipment > assays > next generation sequencing
Investigative techniques and equipment > assays > Single cell sequencing
CSHL Authors:
Communities: CSHL Cancer Center Program > Cancer Genetics and Genomics Program
CSHL labs > Hammell M. lab
CSHL labs > Hicks lab
School of Biological Sciences > Publications
Depositing User: Matthew Dunn
Date: 7 January 2019
Date Deposited: 10 Jan 2019 21:38
Last Modified: 09 Sep 2021 18:36
PMCID: PMC6771363
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