BAS1 has a Myb motif and activates HIS4 transcription only in combination with BAS2

Tice-Baldwin, K., Fink, G. R., Arndt, K. T. (November 1989) BAS1 has a Myb motif and activates HIS4 transcription only in combination with BAS2. Science, 246 (4932). pp. 931-5. ISSN 0036-8075

DOI: 10.1126/science.2683089


The BAS1 and BAS2 proteins are both required for activation of GCN4-independent (basal) HIS4 transcription in yeast. BAS1 has an NH2-terminal region similar to those of the myb proto-oncogene family. BAS1 and BAS2, which contains a homeo box, bound to adjacent sites on the HIS4 promoter. The joint requirement of BAS1 and BAS2 for activation is probably not due to cooperative binding or the transcriptional control of one of the genes by the other. Although BAS1 and BAS2 were both required for activation of HIS4 transcription, BAS1 was not required for BAS2-dependent expression of the secreted acid phosphatases. The transcriptional activators of HIS4 have DNA binding domains that are conserved in evolution (BAS1 = Myb, BAS2 = homeo box, GCN4 = Jun). Their interactions, therefore, may be relevant to the control of gene expression in more complex systems.

Item Type: Paper
Uncontrolled Keywords: Amino Acid Sequence Animals Base Sequence Comparative Study Fungal Proteins/*genetics *Gene Expression Regulation *Genes, Fungal Molecular Sequence Data Proto-Oncogene Proteins/*genetics Proto-Oncogene Proteins c-myb Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S. Saccharomyces cerevisiae/*genetics *Saccharomyces cerevisiae Proteins Sequence Homology, Nucleic Acid *Trans-Activators *Transcription, Genetic
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > transcription
CSHL Authors:
Communities: CSHL labs
Depositing User: Gail Sherman
Date: 17 November 1989
Date Deposited: 06 Jun 2017 15:39
Last Modified: 06 Jun 2017 15:39
Related URLs:

Actions (login required)

Administrator's edit/view item Administrator's edit/view item
CSHL HomeAbout CSHLResearchEducationNews & FeaturesCampus & Public EventsCareersGiving