Dyson, N., Buchkovich, K., Whyte, P., Harlow, E. (July 1989) The cellular 107K protein that binds to adenovirus E1A also associates with the large T antigens of SV40 and JC virus. Cell, 58 (2). pp. 249-55. ISSN 0092-8674
Abstract
The association between the retinoblastoma protein (p105-RB) and either the large T antigen of SV40 or the E1A proteins of adenovirus is thought to be an important step in transformation by these viral oncogenes. E1A and large T antigen share a small region of amino acid homology that is necessary for high affinity binding with p105-RB. Mutations of this homology region were shown to reduce drastically the frequency of transformation mediated by the E1A or large T oncogenes. Previously, this small region in E1A was shown to be sufficient for interaction with a second cellular protein of 107,000 daltons (107K). Here we show that in human cells, the large T antigens of SV40 or JC virus also form complexes with 107K. Demonstration of complexes between 107K and the large T antigens of SV40 and JC virus suggests that these associations may represent another component of a common mechanism for transformation between adenoviruses and polyoma viruses.
| Item Type: | Paper |
|---|---|
| Uncontrolled Keywords: | Adenovirus Early Proteins Antigens, Polyomavirus Transforming/*immunology Antigens, Viral, Tumor/*immunology Cell Line, Transformed Gene Expression Regulation Humans JC Virus/*immunology Oncogene Proteins, Viral/*metabolism Phosphoproteins/metabolism Polyomavirus/*immunology Proteins/immunology/*metabolism Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S. Retinoblastoma/genetics/metabolism Retinoblastoma Protein Simian virus 40/*immunology |
| Subjects: | bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types organism description > virus |
| CSHL Authors: | |
| Communities: | CSHL labs |
| Depositing User: | Gail Sherman |
| Date: | 28 July 1989 |
| Date Deposited: | 31 Jul 2017 18:34 |
| Last Modified: | 31 Jul 2017 18:34 |
| Related URLs: | |
| URI: | https://repository.cshl.edu/id/eprint/34841 |
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