Muscle- and Skin-Derived Cues Jointly Orchestrate Patterning of Somatosensory Dendrites

Diaz-Balzac, C. A., Rahman, M., Lazaro-Pena, M. I., Martin Hernandez, L. A., Salzberg, Y., Aguirre-Chen, C., Kaprielian, Z., Bulow, H. E. (September 2016) Muscle- and Skin-Derived Cues Jointly Orchestrate Patterning of Somatosensory Dendrites. Curr Biol, 26 (17). pp. 2379-2387. ISSN 1879-0445 (Electronic)0960-9822 (Linking)

URL: https://www.ncbi.nlm.nih.gov/pubmed/27451901
DOI: 10.1016/j.cub.2016.07.008

Abstract

Sensory dendrite arbors are patterned through cell-autonomously and non-cell-autonomously functioning factors [1-3]. Yet, only a few non-cell-autonomously acting proteins have been identified, including semaphorins [4, 5], brain-derived neurotrophic factors (BDNFs) [6], UNC-6/Netrin [7], and the conserved MNR-1/Menorin-SAX-7/L1CAM cell adhesion complex [8, 9]. This complex acts from the skin to pattern the stereotypic dendritic arbors of PVD and FLP somatosensory neurons in Caenorhabditis elegans through the leucine-rich transmembrane receptor DMA-1/LRR-TM expressed on PVD neurons [8, 9]. Here we describe a role for the diffusible C. elegans protein LECT-2, which is homologous to vertebrate leukocyte cell-derived chemotaxin 2 (LECT2)/Chondromodulin II. LECT2/Chondromodulin II has been implicated in a variety of pathological conditions [10-13], but the developmental functions of LECT2 have remained elusive. We find that LECT-2/Chondromodulin II is required for development of PVD and FLP dendritic arbors and can act as a diffusible cue from a distance to shape dendritic arbors. Expressed in body-wall muscles, LECT-2 decorates neuronal processes and hypodermal cells in a pattern similar to the cell adhesion molecule SAX-7/L1CAM. LECT-2 functions genetically downstream of the MNR-1/Menorin-SAX-7/L1CAM adhesion complex and upstream of the DMA-1 receptor. LECT-2 localization is dependent on SAX-7/L1CAM, but not on MNR-1/Menorin or DMA-1/LRR-TM, suggesting that LECT-2 functions as part of the skin-derived MNR-1/Menorin-SAX-7/L1CAM adhesion complex. Collectively, our findings suggest that LECT-2/Chondromodulin II acts as a muscle-derived, diffusible cofactor together with a skin-derived cell adhesion complex to orchestrate the molecular interactions of three tissues during patterning of somatosensory dendrites.

Item Type: Paper
Subjects: organs, tissues, organelles, cell types and functions > tissues types and functions > somatosensory cortex
CSHL Authors:
Communities: CSHL labs > Hammell C. lab
Depositing User: Matt Covey
Date: 12 September 2016
Date Deposited: 07 Oct 2016 14:39
Last Modified: 07 Oct 2016 14:39
PMCID: PMC5021591
Related URLs:
URI: https://repository.cshl.edu/id/eprint/33630

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