Chen, L. B., Murray, A., Segal, R. A., Bushnell, A., Walsh, M. L. (June 1978) Studies on intercellular LETS glycoprotein matrices. Cell, 14 (2). pp. 377-91. ISSN 0092-8674 (Print)0092-8674 (Linking)
Abstract
Intercellular matrices secreted by chick embryo fibroblasts in culture were studied by scanning electron microscopy. Cell-cell contact is a prerequisite for the expression of such matrices. The smallest fiber detected by transmission electron microscopy is 5--10 nm in diameter. These matrix fibers tend to cluster to form bundles. Immunofluorescence and immunoferritin procedures reveal that LETS protein is one of the components of the matrices. The matrices are isolated from other cellular organelles by detergent treatment. More than 90% of the proteins in cell-free matrices are LETS protein, suggesting that the matrices are probably made of only one component--LETS protein. Since the solubilization of matrices requires beta-mercaptoethanol, LETS protein matrices may be the first known polymer system in nature to use disulfide linkage as an intermolecular polymerization vehicle. Collagen does not appear to be involved in such matrices. The LETS protein matrix supports the morphological conversion of rounded cells into spindle-shaped, and also promotes myoblast fusion. It does not, however, exert an effect upon cell growth, the rate of glucose uptake or protease production.
| Item Type: | Paper |
|---|---|
| Uncontrolled Keywords: | *Cell Count Cell Division Cell Fusion Cell-Free System Cells, Cultured Glucose/metabolism Glycoproteins/*metabolism Membrane Proteins/*metabolism Mercaptoethanol/pharmacology Microscopy, Electron, Scanning Peptide Hydrolases/biosynthesis |
| Subjects: | organs, tissues, organelles, cell types and functions > cell types and functions > cell types > fibroblasts organs, tissues, organelles, cell types and functions > cell types and functions > cell types > fibroblasts organs, tissues, organelles, cell types and functions > cell types and functions > cell types > fibroblasts bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > matrix protein |
| CSHL Authors: | |
| Communities: | CSHL labs |
| Depositing User: | Matt Covey |
| Date: | June 1978 |
| Date Deposited: | 25 Aug 2016 12:19 |
| Last Modified: | 25 Aug 2016 12:19 |
| Related URLs: | |
| URI: | https://repository.cshl.edu/id/eprint/33337 |
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