Kucherlapati, R., Hwang, S. P., Shimizu, N., McDougall, J. K., Botchan, M. R. (September 1978) Another chromosomal assignment for a simian virus 40 integration site in human cells. Proc Natl Acad Sci U S A, 75 (9). pp. 4460-4. ISSN 0027-8424 (Print)0027-8424 (Linking)
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Abstract
Somatic cell hybrids derived from fusion of GM637, a human cell line transformed by simian virus 40, and mouse B82 cells were examined for simian virus 40 T antigen, V antigen, and viral DNA. All hybrid cell lines that contained viral DNA were T-antigen positive. Cells that did not have viral DNA were T-antigen negative. We determined that there is a single viral insertion in these hybrid cells. Correlation of T-antigen expression and viral DNA with the partial complements of the human genome retained in the hybrids shwed that the inserted viral genome is in human chromosome 8. The integrated viral DNA is stable; free viral DNA found in GM637 does not insert at other potential sites in the human genome.
| Item Type: | Paper |
|---|---|
| Uncontrolled Keywords: | Antigens, Viral/analysis Cell Line *Chromosomes, Human, 6-12 and X DNA, Viral/analysis/*metabolism Humans Hybrid Cells/microbiology Simian virus 40/*genetics/immunology Virus Replication |
| Subjects: | bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > chromosome bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > chromosomes, structure and function > chromosome organism description > virus > SV40 |
| CSHL Authors: | |
| Communities: | CSHL labs |
| Depositing User: | Matt Covey |
| Date: | September 1978 |
| Date Deposited: | 13 Dec 2016 17:07 |
| Last Modified: | 09 Nov 2017 21:02 |
| PMCID: | PMC336135 |
| Related URLs: | |
| URI: | https://repository.cshl.edu/id/eprint/33303 |
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