Ito, J., Roberts, R. J. (January 1979) Unusual base sequence arrangement in phage phi 29 DNA. Gene, 5 (1). pp. 1-7. ISSN 0378-1119 (Print)0378-1119 (Linking)
Abstract
Susceptibility of Bacillus subtilis phage phi 29 DNA to 34 different restriction endoculceases was determined. Three enzymes, BglI, XbaI and BstEII, were found to cleave phi 29 DNA only once at specific sites. The sites of these single cleavages have been mapped. Thirteen enzymes did not cut phi 29 DNA. phi 29 HindIII DNA fragments inserted into pBR313 plasmid and propagated in Escherichia coli, were resistant to these restriction endonucleases. This result suggests that the insusceptibility is due to the absence of the nucleotide sequences on phi 29 recognized by the enzymes, and not to the presence of modified nucleotides.
| Item Type: | Paper |
|---|---|
| Uncontrolled Keywords: | Bacillus subtilis Bacteriophages/*genetics Base Sequence DNA Restriction Enzymes/metabolism DNA, Recombinant DNA, Viral/*analysis Escherichia coli/genetics Plasmids |
| Subjects: | organs, tissues, organelles, cell types and functions > cell types and functions > cell types > bacteriophage organs, tissues, organelles, cell types and functions > cell types and functions > cell types > bacteriophage organs, tissues, organelles, cell types and functions > cell types and functions > cell types > bacteriophage organism description > bacteria > escherichia coli bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > restriction enzyme |
| CSHL Authors: | |
| Communities: | CSHL labs > Roberts lab |
| Depositing User: | Matt Covey |
| Date: | January 1979 |
| Date Deposited: | 25 May 2016 19:19 |
| Last Modified: | 25 May 2016 19:19 |
| Related URLs: | |
| URI: | https://repository.cshl.edu/id/eprint/32693 |
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