Origin-defective mutants of SV40

Gluzman, Y., Frisque, R. J., Sambrook, J. (1980) Origin-defective mutants of SV40. Cold Spring Harb Symp Quant Biol, 44 Pt . pp. 293-300. ISSN 0091-7451 (Print)0091-7451 (Linking)

URL: http://www.ncbi.nlm.nih.gov/pubmed/6253143
DOI: 10.1101/SQB.1980.044.01.033


We have described a new technique for the isolation and propagation of nonconditionally lethal mutants. We have used this method to generate mutants of SV40 that contain a defective origin of DNA replication. Using these mutants, we have established the following: (1) SV40 DNA replication and early transcription are functionally separate. (2) A functional viral origin of DNA replication is not necessary for the maintenance of transformation. (3) The lack of the origin of SV40 DNA replication does not affect the efficiency of transformation when nonpermissive cells are transfected by DNA using the calcium technique.

Item Type: Paper
Uncontrolled Keywords: Animals Antibodies, Viral Antigens, Neoplasm Antigens, Viral *Cell Transformation, Viral Cells, Cultured DNA, Recombinant DNA, Viral/genetics Mutation Plasmids Rats Simian virus 40/*genetics *Virus Replication
Subjects: organs, tissues, organelles, cell types and functions > cell types and functions > cell functions > cell transformation
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > mutations
organism description > virus > SV40
CSHL Authors:
Communities: CSHL labs
Depositing User: Matt Covey
Date: 1980
Date Deposited: 23 May 2016 19:12
Last Modified: 23 May 2016 19:12
Related URLs:
URI: https://repository.cshl.edu/id/eprint/32686

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