Tokiwa, G., Tyers, M., Volpe, T., Futcher, B. (September 1994) Inhibition of G1 cyclin activity by the Ras/cAMP pathway in yeast. Nature, 371 (6495). pp. 342-5. ISSN 0028-0836 (Print)
Abstract
In the yeast Saccharomyces cerevisiae, commitment to cell division (Start) requires growth to a critical cell size. The G1 cyclins Cln1, Cln2 and Cln3 activate the Cdc28 protein kinase and are rate-limiting activators of Start. When glucose is added to cells growing in a poor carbon source, the critical cell size required for Start is reset from a small to a large size. In yeast, glucose acts through Ras proteins to stimulate adenylyl cyclase, activating the three cyclic AMP-dependent protein kinases Tpk1, Tpk2 and Tpk3 (refs 8, 9). We find that stimulation of the Ras/cAMP pathway represses expression of CLN1, CLN2 and co-regulated genes, inhibiting Start. This helps explain the increase in critical size when cells are shifted from poor to rich medium. This connection between the molecules controlling growth (Ras/cAMP) and those controlling division (cyclins) helps explain how division is co-ordinated with growth.
| Item Type: | Paper |
|---|---|
| Uncontrolled Keywords: | Cell Division/genetics/physiology Cyclic AMP/ metabolism Cyclic AMP-Dependent Protein Kinases/metabolism Cyclins/ antagonists & inhibitors/metabolism Fungal Proteins/ metabolism G1 Phase/genetics/physiology Gene Expression Regulation, Fungal Glucose/metabolism Mutation Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S. Saccharomyces cerevisiae/ cytology/genetics/metabolism Signal Transduction ras Proteins |
| Subjects: | bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > Cyclins bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > transcription factor > Cyclic AMP bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > G protein > Ras organism description > yeast |
| CSHL Authors: | |
| Communities: | CSHL labs > Futcher lab |
| Depositing User: | Matt Covey |
| Date: | 22 September 1994 |
| Date Deposited: | 05 May 2015 20:51 |
| Last Modified: | 05 May 2015 20:51 |
| Related URLs: | |
| URI: | https://repository.cshl.edu/id/eprint/31454 |
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