Developmental and tissue-specific regulation of mouse telomerase and telomere length

Prowse, K. R., Greider, C. W. (May 1995) Developmental and tissue-specific regulation of mouse telomerase and telomere length. Proc Natl Acad Sci U S A, 92 (11). pp. 4818-22. ISSN 0027-8424 (Print)

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Telomere shortening and telomerase activation in human somatic cells have been implicated in cell immortalization and cellular senescence. To further study the role of telomerase in immortalization, we assayed telomere length and telomerase activity in primary mouse fibroblasts, in spontaneously immortalized cell clones, and in mouse tissues. In the primary cell cultures, telomere length decreased with increased cell doublings and telomerase activity was not detected. In contrast, in spontaneously immortalized clones, telomeres were maintained at a stable length and telomerase activity was present. To determine if telomere shortening occurs in vivo, we assayed for telomerase and telomere length in tissues from mice of different ages. Telomere length was similar among different tissues within a newborn mouse, whereas telomere length differed between tissues in an adult mouse. These findings suggest that there is tissue-specific regulation of mouse telomerase during development and aging in vivo. In contrast to human tissues, most mouse tissues had active telomerase. The presence of telomerase in these tissues may reflect the ease of immortalization of primary mouse cells relative to human cells in culture.

Item Type: Paper
Uncontrolled Keywords: Animals Animals, Newborn Brain/enzymology Cell Aging Cell Division Cells, Cultured Comparative Study DNA Nucleotidylexotransferase/ metabolism Fibroblasts/cytology/enzymology Humans Kinetics Liver/enzymology Male Mice Mice, Inbred BALB C Mice, Inbred Strains Muridae Organ Specificity Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S. Skin/ cytology/ enzymology Species Specificity Spleen/enzymology Telomere Testis/enzymology
Subjects: organism description > animal > mammal > rodent > mouse
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > enzymes > telomerase
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > telomeres
organs, tissues, organelles, cell types and functions > tissues types and functions
CSHL Authors:
Communities: CSHL labs
Depositing User: Jessica Koos
Date: 23 May 1995
Date Deposited: 11 Aug 2014 18:22
Last Modified: 09 Nov 2017 19:30
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