Kimura, K., Hirano, M., Kobayashi, R., Hirano, T.
(October 1998)
Phosphorylation and activation of 13S condensin by Cdc2 in vitro.
Science, 282 (5388).
pp. 487-90.
ISSN 0036-8075 (Print)
Abstract
13S condensin is a multisubunit protein complex essential for mitotic chromosome condensation in Xenopus egg extracts. Purified 13S condensin introduces positive supercoils into DNA in the presence of topoisomerase I and adenosine triphosphate in vitro. The supercoiling activity of 13Scondensin was regulated by mitosis-specific phosphorylation. Immunodepletion, in vitro phosphorylation, and peptide-mapping experiments indicated that Cdc2 is likely to be the kinase that phosphorylates and activates 13S condensin. Multiple Cdc2 phosphorylation sites are clustered in the carboxyl-terminal domain of the XCAP-D2 (Xenopus chromosome-associated polypeptide D2) subunit. These results suggest that phosphorylation of 13Scondensin by Cdc2 may trigger mitotic chromosome condensation in vitro.
| Item Type: |
Paper
|
| Uncontrolled Keywords: |
Adenosinetriphosphatase/chemistry/ metabolism
Amino Acid Sequence
Animals
CDC2 Protein Kinase/ metabolism
Chromosomes/chemistry/ metabolism
DNA, Circular/chemistry/metabolism
DNA, Superhelical/ chemistry
DNA-Binding Proteins/chemistry/ metabolism
Enzyme Activation
Interphase
Mitosis
Molecular Sequence Data
Multiprotein Complexes
Nucleic Acid Conformation
Peptide Mapping
Phosphorylation
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S.
Xenopus |
| Subjects: |
bioinformatics > genomics and proteomics > genetics & nucleic acid processing
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > chromosome
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > chromosomes, structure and function > chromosome
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > DNA binding protein
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein expression > phosphorylation
organism description > animal > Frog > xenopus |
| CSHL Authors: |
|
| Communities: |
CSHL labs > Hirano lab
CSHL labs > Kobayashi lab |
| Depositing User: |
Kathleen Darby
|
| Date: |
16 October 1998 |
| Date Deposited: |
01 May 2014 19:02 |
| Last Modified: |
01 May 2014 19:02 |
| Related URLs: |
|
| URI: |
https://repository.cshl.edu/id/eprint/29903 |
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