McGinn, J., Czech, B. (2014) Small RNA library construction for high-throughput sequencing. Methods in Molecular Biology, 1093. pp. 195-208. ISSN 10643745 (ISSN); 9781627036931 (ISBN)
Abstract
Since their discovery about 20 years ago, small RNAs have been shown to play a critical role in a myriad of biological processes. The greater availability of high-throughput sequencing has been invaluable to furthering our understanding of small RNAs as regulatory molecules. In particular, these sequencing technologies have been crucial in understanding the role of small RNAs in reproductive tissues, where millions of individual sequences are generated. In this context, high-throughput sequencing provides the requisite level of resolution that other procedures, like northern blotting, would not be able to achieve. Here, we describe a protocol for the preparation of small RNA libraries for sequencing using the Solexa/Illumina technology. © 2014 Springer Science+Business Media, LLC.
| Item Type: | Paper |
|---|---|
| Uncontrolled Keywords: | Cloning Endo-siRNA High-throughput sequencing miRNAs piRNAs Small RNA libraries |
| Subjects: | bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification Investigative techniques and equipment > assays > next generation sequencing bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > sRNA |
| CSHL Authors: | |
| Communities: | CSHL labs > Hannon lab |
| Depositing User: | Matt Covey |
| Date: | 2014 |
| Date Deposited: | 04 Feb 2014 15:47 |
| Last Modified: | 04 Feb 2014 15:47 |
| Related URLs: | |
| URI: | https://repository.cshl.edu/id/eprint/29469 |
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