Zhao, X. Y., Pendergrast, P. S., Hernandez, N. (March 2001) A positioned nucleosome on the human U6 promoter allows recruitment of SNAP(c) by the Oct-1 POU domain. Molecular Cell, 7 (3). pp. 539-549. ISSN 1097-2765
Abstract
The human snRNA promoters contain a proximal sequence element (PSE) required for basal transcription and a distal sequence element (DSE) required for activated transcription. The PSE recruits the multisubunit factor SNAP,, whereas the DSE recruits Oct-1. Oct-1 and SNAP, bind cooperatively to DNA when their respective binding sites are moved into proximity through a mechanism that involves a defined protein-protein contact. Here, we show that on the natural U6 promoter, cooperative binding of Oct-1 and SNAP, is mediated by a positioned nucleosome that resides between the DSE and the PSE. This cooperative binding requires the same protein-protein contact as cooperative binding to closely spaced sites on naked DNA and mediates transcription activation.
| Item Type: | Paper |
|---|---|
| Uncontrolled Keywords: | RNA-POLYMERASE-II SMALL NUCLEAR-RNA DNA-BINDING DOMAIN ACTIVATES TRANSCRIPTION GENE-TRANSCRIPTION COMPLEX SNAP(C) DISTAL PROMOTER SNRNA PROMOTERS CHROMATIN REGION |
| Subjects: | bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > nucleosome bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > DNA expression > promoter |
| CSHL Authors: | |
| Communities: | CSHL labs > Hernandez lab |
| Depositing User: | Matt Covey |
| Date: | March 2001 |
| Date Deposited: | 15 Jan 2014 21:13 |
| Last Modified: | 15 Jan 2014 21:13 |
| Related URLs: | |
| URI: | https://repository.cshl.edu/id/eprint/29335 |
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