Nakayama, J., Rice, J. C., Strahl, B. D., Allis, C. D., Grewal, S. I. S.
(April 2001)
Role of histone H3 lysine 9 methylation in epigenetic control of heterochromatin assembly.
Science, 292 (5514).
pp. 110-113.
ISSN 0036-8075
Abstract
The assembly of higher order chromatin structures has been Linked to the covalent modifications of histone tails. We provide in vivo evidence that lysine 9 of histone H3 (H3 Lys(9)) is preferentially methylated by the Clr4 protein at heterochromatin-associated regions in fission yeast. Both the conserved chromo- and SET domains of Clr4 are required for H3 Lys(9) methylation in vivo. Localization of Swi6, a homolog of Drosophila HP1, to heterochomatic regions is dependent on H3 Lysg methylation. Moreover, an H3-specific deacetylase Clr3 and a beta -propeller domain protein Rik1 are required for H3 Lys(9) methylation by Clr4 and Swi6 Localization. These data define a conserved pathway wherein sequential histone modifications establish a "histone code" essential for the epigenetic inheritance of heterochromatin assembly.
| Item Type: |
Paper
|
| Uncontrolled Keywords: |
FISSION YEAST
CHROMOSOME SEGREGATION
PROTEIN
ACETYLATION
CENTROMERE
CHROMATIN
DOMAINS
INHERITANCE
DROSOPHILA
MUTATIONS |
| Subjects: |
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > Chromatin dynamics
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > epigenetics
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > epigenetics
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > histone
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein methylation > histone methylation |
| CSHL Authors: |
|
| Communities: |
CSHL labs > Grewal lab |
| Depositing User: |
Matt Covey
|
| Date: |
April 2001 |
| Date Deposited: |
17 Jan 2014 20:04 |
| Last Modified: |
17 Jan 2014 20:04 |
| Related URLs: |
|
| URI: |
https://repository.cshl.edu/id/eprint/29275 |
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