The essential pre-mRNA splicing factor SF2 influences 5' splice site selection by activating proximal sites

Krainer, A. R., Conway, G. C., Kozak, D. (July 1990) The essential pre-mRNA splicing factor SF2 influences 5' splice site selection by activating proximal sites. Cell, 62 (1). pp. 35-42. ISSN 0092-8674

Abstract

SF2 is a 33 kd protein factor required for 5′ splice site cleavage and lariat formation during pre-mRNA splicing in HeLa cell extracts. In addition to its essential role in constitutive splicing, SF2 can strongly influence 5′ splice site selection. When pre-mRNAs containing multiple cis-competing 5′ splice sites are spliced in vitro, high concentrations of purified SF2 promote the use of the 5′ splice site closest to the 3′ splice site. However, SF2 discriminates properly between authentic and cryptic splice sites. These effects of SF2 on splice site selection may reflect the cellular mechanisms that prevent exon skipping and ensure the accuracy of splicing. In addition, alterations in the concentration or activity of SF2, and of other general splicing factors, may serve to regulate alternative splicing in vivo.

Item Type:	Paper
Subjects:	bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification bioinformatics > genomics and proteomics > genetics & nucleic acid processing bioinformatics > genomics and proteomics bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > pre-mRNA bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > splicing factor
CSHL Authors:	Krainer, Adrian R.
Communities:	CSHL labs > Krainer lab
Depositing User:	Matt Covey
Date:	July 1990
Date Deposited:	10 Dec 2013 20:38
Last Modified:	10 Dec 2013 20:38
Related URLs:	Publisher
URI:	https://repository.cshl.edu/id/eprint/28961

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