Burns, C. G., Ohi, R., Krainer, A. R., Gould, K. L. (November 1999) Evidence that Myb-related CDC5 proteins are required for pre-mRNA splicing. Proceedings of the National Academy of Sciences of the United States of America, 96 (24). pp. 13789-13794. ISSN 0027-8424
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Abstract
The conserved CDC5 family of Myb-related proteins performs an essential function in cell cycle control at G(2)/M. Although c-Myb and many Myb-related proteins act as transcription factors, herein, we implicate CDC5 proteins in pre-mRNA splicing. Mammalian CDC5 colocalizes with pre-mRNA splicing factors in the nuclei of mammalian cells, associates with core components of the splicing machinery in nuclear extracts, and interacts with the spliceosome throughout the splicing reaction in vitro. Furthermore, genetic depletion of the homolog of CDC5 in Saccharomyces cerevisiae, CEF1. blocks the first step of pre-mRNA processing in vivo. These data provide evidence that eukaryotic cells require CDC5 proteins for pre-mRNA splicing.
| Item Type: | Paper |
|---|---|
| Uncontrolled Keywords: | yeast saccharomyces-cerevisiae cell-division cycle schizosaccharomyces-pombe fission yeast gene encodes mutants nucleus dna organization |
| Subjects: | bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification bioinformatics > genomics and proteomics > genetics & nucleic acid processing bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > pre-mRNA bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > RNA splicing organism description > yeast |
| CSHL Authors: | |
| Communities: | CSHL labs > Krainer lab |
| Depositing User: | Matt Covey |
| Date: | November 1999 |
| Date Deposited: | 11 Dec 2013 15:03 |
| Last Modified: | 10 Sep 2019 19:38 |
| PMCID: | PMC24143 |
| Related URLs: | |
| URI: | https://repository.cshl.edu/id/eprint/28931 |
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