High-frequency switching in Candida strains isolated from vaginitis patients

Soll, D. R., Langtimm, C. J., McDowell, J., Hicks, J., Galask, R. (1987) High-frequency switching in Candida strains isolated from vaginitis patients. Journal of Clinical Microbiology, 25 (9). pp. 1611-1622. ISSN 00951137 (ISSN)

URL: http://www.ncbi.nlm.nih.gov/pubmed/2821060


High-frequency switching and strain variability at the site of infection was assessed in 11 patients with acute Candida albicans vaginitis. By cloning cells directly from the site of infection, it was demonstrated that 4 of the 11 isolates contained multiple-switch phenotypes at the site of infection and that 9 of the 11 isolates were in a high-frequency mode of switching (10-2 to 10-3). Isolates could be separated into four general categories of switching repertoires. To demonstrate that multiple phenotypes at the site of a single infection represented the same strain, EcoRI digests of total cell DNA were separated on agarose gels, and Southern hybridization patterns with two cloned midrepeat sequences were compared.

Item Type: Paper
Uncontrolled Keywords: cell dna candida diagnosis female genital system fungus human phenotype priority journal transposition vaginitis Candida albicans Candidiasis, Vulvovaginal Cloning, Molecular Deoxyribonuclease EcoRI DNA Restriction Enzymes DNA, Fungal Electrophoresis, Agar Gel Female Humans Nucleic Acid Hybridization Vagina
Subjects: diseases & disorders
organism description > yeast
CSHL Authors:
Communities: CSHL labs > Hicks lab
Depositing User: Matt Covey
Date: 1987
Date Deposited: 09 Aug 2013 20:56
Last Modified: 12 Aug 2013 14:26
Related URLs:
URI: https://repository.cshl.edu/id/eprint/28509

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