Neural cell adhesion molecule-mediated fyn activation promotes GABAergic synapse maturation in postnatal mouse cortex

Chattopadhyaya, B., Baho, E., Huang, Z. J., Schachner, M., Di Cristo, G. (2013) Neural cell adhesion molecule-mediated fyn activation promotes GABAergic synapse maturation in postnatal mouse cortex. Journal of Neuroscience, 33 (14). pp. 5957-68. ISSN 0270-6474

Preview

PDF (Paper) Huang J Neuroscience 2013.pdf - Published Version Download (7MB) | Preview

Abstract

GABAergic basket interneurons form perisomatic synapses, which are essential for regulating neural networks, and their alterations are linked to various cognitive dysfunction. Maturation of basket synapses in postnatal cortex is activity dependent. In particular, activity-dependent downregulation of polysialiac acid carried by the neural cell adhesion molecule (NCAM) regulates the timing of their maturation. Whether and how NCAM per se affects GABAergic synapse development is unknown. Using single-cell genetics to knock out NCAM in individual basket interneurons in mouse cortical slice cultures, at specific developmental time periods, we found that NCAM loss during perisomatic synapse formation impairs the process of basket cell axonal branching and bouton formation. However, loss of NCAM once the synapses are already formed did not show any effect. We further show that NCAM120 and NCAM140, but not the NCAM180 isoform, rescue the phenotype. Finally, we demonstrate that a dominant-negative form of Fyn kinase mimics, whereas a constitutively active form of Fyn kinase rescues, the effects of NCAM knockdown. Altogether, our data suggest that NCAM120/NCAM140-mediated Fyn activation promotes GABAergic synapse maturation in postnatal cortex.

Item Type:	Paper
Subjects:	bioinformatics > genomics and proteomics > small molecules > GABA organs, tissues, organelles, cell types and functions > cell types and functions > cell types > neocortical interneurons organs, tissues, organelles, cell types and functions > cell types and functions > cell types > neocortical interneurons organs, tissues, organelles, cell types and functions > cell types and functions > cell types > neocortical interneurons organs, tissues, organelles, cell types and functions > cell types and functions > cell types > neurons > neocortical interneurons organs, tissues, organelles, cell types and functions > cell types and functions > cell types > neurons > neocortical interneurons organs, tissues, organelles, cell types and functions > cell types and functions > cell types > neurons > neocortical interneurons organs, tissues, organelles, cell types and functions > tissues types and functions > cerebral cortex organism description > animal > mammal > rodent > mouse organs, tissues, organelles, cell types and functions > tissues types and functions > neural networks bioinformatics > genomics and proteomics > small molecules organs, tissues, organelles, cell types and functions > sub-cellular tissues: types and functions > synapse
CSHL Authors:	Huang, Z. Josh
Communities:	CSHL labs > Huang lab
Depositing User:	Matt Covey
Date:	2013
Date Deposited:	22 May 2013 19:09
Last Modified:	19 Jul 2021 13:31
PMCID:	PMC6618917
Related URLs:	Publisher
URI:	https://repository.cshl.edu/id/eprint/28307

Actions (login required)

Administrator's edit/view item